Novel electrospun gelatin/oxycellulose nanofibers as a suitable platform for lung disease modeling.

Veronika Švachová, Lucy Vojtová, David Pavliňák, Libor Vojtek, Veronika Sedláková, Pavel Hyršl, Milan Alberti, Josef Jaroš, Aleš Hampl, Josef Jančář

Journal: Materials science & engineering. C, Materials for biological applications 2017;67():493-501

PMID: 27287147

Full Text on Elsevier Science View on PubMed

Abstract

Novel hydrolytically stable gelatin nanofibers modified with sodium or calcium salt of oxycellulose were prepared by electrospinning method. The unique inhibitory effect of these nanofibers against Escherichia coli bacteria was examined by luminometric method. Biocompatibility of these gelatin/oxycellulose nanofibers with eukaryotic cells was tested using human lung adenocarcinoma cell line NCI-H441. Cells firmly adhered to nanofiber surface, as determined by scanning electron microscopy, and no signs of cell dying were detected by fluorescent live/dead assay. We propose that the newly developed gelatin/oxycellulose nanofibers could be used as promising scaffold for lung disease modeling and anti-cancer drug testing.

Address: Institute of Materials Chemistry, Brno University of Technology, Czech Republic. Electronic address: [email protected].; CEITEC - Central European Institute of Technology, Brno University of Technology, Czech Republic; SCITEG, a.s., Brno, Czech Republic.; Department of Physical Electronics, Masaryk University, Czech Republic.; Institute of Experimental Biology, Masaryk University, Czech Republic.; Department of Histology and Embryology, Masaryk University, Czech Republic; International Clinical Research, St. Anne's University Hospital, Brno, Czech Republic.; Institute of Materials Chemistry, Brno University of Technology, Czech Republic; CEITEC - Central European Institute of Technology, Brno University of Technology, Czech Republic; SCITEG, a.s., Brno, Czech Republic.

DOI: 10.1016/j.msec.2016.05.059

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MeSH Terms: Adenocarcinoma, Cell Line, Tumor, Cellulose, Oxidized, Escherichia coli, Gelatin, Humans, Lung Neoplasms, Nanofibers