Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae.

Jiantao Zhang, Chaochen Wu, Jiayuan Sheng, Xueyang Feng

Journal: Molecular bioSystems 2017;12(5):1432-5

PMID: 27008988

Full Text on Royal Society of Chemistry View on PubMed

Abstract

We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors. The innate DFR1 gene in the folate synthesis pathway was found to play pivotal roles in 5-hydroxytryptophan synthesis.

Address: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. [email protected] and Department of Pharmacology & Toxicology, University of Arizona, Tucson, AZ 85721, USA.; Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. [email protected].

DOI: 10.1039/c5mb00888c

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https://doi.org/10.1039/c5mb00888c

MeSH Terms: 5-Hydroxytryptophan, Biopterins, Folic Acid, Gene Expression Regulation, Fungal, Phenylalanine Hydroxylase, Saccharomyces cerevisiae, Tryptophan Hydroxylase