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Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists.

Satomi Kanno, Laura Cuyas, Hélène Javot, Richard Bligny, Elisabeth Gout, Thibault Dartevelle, Mohamed Hanchi, Tomoko M Nakanishi, Marie-Christine Thibaud, Laurent Nussaume

Journal: Plant & cell physiology 2017;57(4):690-706

PMID: 26865660

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Abstract

Phosphate (Pi) is a macronutrient that is essential for plant life. Several regulatory components involved in Pi homeostasis have been identified, revealing a very high complexity at the cellular and subcellular levels. Determining the Pi content in plants is crucial to understanding this regulation, and short real-time(33)Pi uptake imaging experiments have shown Pi movement to be highly dynamic. Furthermore, gene modulation by Pi is finely controlled by localization of this ion at the tissue as well as the cellular and subcellular levels. Deciphering these regulations requires access to and quantification of the Pi pool in the various plant compartments. This review presents the different techniques available to measure, visualize and trace Pi in plants, with a discussion of the future prospects.

© The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: [email protected].

Address: Commissariat à l'Energie Atomique (CEA), Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Biologie du Developpement des Plantes; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 7265 Biologie Vegetale & Microbiologie Environnementale; Aix-Marseille Universite, Saint-Paul-lez-Durance, F-13108, France Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi, 1-1-1, Bunkyo-ku, Tokyo, 113-8657 Japan Biotechnology Research Center, the University of Tokyo, Yayoi, 1-1-1, Bunkyo-ku, Tokyo, 113-8657 Japan.; Commissariat à l'Energie Atomique (CEA), Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Biologie du Developpement des Plantes; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 7265 Biologie Vegetale & Microbiologie Environnementale; Aix-Marseille Universite, Saint-Paul-lez-Durance, F-13108, France.; CEA, Institut de Recherches en Technologies et Sciences pour le Vivant, Laboratoire de Physiologie Cellulaire & Végétale, Unité Mixte de Recherche 5168, CNRS, Université Grenoble Alpes, Institut National de la Recherche Agronomique (INRA), CEA, Grenoble, F-38054, France.; Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi, 1-1-1, Bunkyo-ku, Tokyo, 113-8657 Japan.; Commissariat à l'Energie Atomique (CEA), Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Biologie du Developpement des Plantes; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 7265 Biologie Vegetale & Microbiologie Environnementale; Aix-Marseille Universite, Saint-Paul-lez-Durance, F-13108, France [email protected].
DOI: 10.1093/pcp/pcv208

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MeSH Terms: Biosensing Techniques, Chromatography, Electrophoresis, Genetic Markers, Magnetic Resonance Spectroscopy, Mass Spectrometry, Phosphates, Phosphorus Isotopes, Plants
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