The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p.

X Le Goff, F Motegi, E Salimova, I Mabuchi, V Simanis

Journal: Journal of cell science 2001;113 Pt 23():4157-63

PMID: 11069761

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Abstract

In order to identify additional components important for cell division in the fission yeast Schizosaccharomyces pombe we have screened a bank of conditional cold-sensitive mutants for cytokinesis defects. One of these mutants showed a delay in cell cleavage, and strong genetic interactions with other genes implicated in medial ring formation. Cloning of the corresponding gene indicates that it encodes a protein with significant homology to the regulatory light chain of non-muscle myosins. We have named the gene rlc1 (regulatory light chain 1). The gene is not essential for division, but null mutants display a cell cleavage defect and form an aberrant F-actin ring. Two myosin-II heavy chains have been identified in fission yeast: Co-immunoprecipitation experiments indicate that rlc1p associates more strongly with myo3p than myo2p.

Address: Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), Switzerland.

DOI: 10.1242/jcs.113.23.4157

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MeSH Terms: Amino Acid Sequence, Carrier Proteins, Cloning, Molecular, Fungal Proteins, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Reporter, Green Fluorescent Proteins, Indicators and Reagents, Luminescent Proteins, Molecular Sequence Data, Mutagenesis, Myosin Heavy Chains, Myosin Light Chains, Myosin Type II, Myosin Type V, Myosins, Nerve Tissue Proteins, Phenotype, Phosphorylation, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins