Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.

D E Korzhevskii, E G Sukhorukova, O V Kirik, I P Grigorev

Journal: European journal of histochemistry : EJH 2016;59(3):2530

PMID: 26428887

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Abstract

Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF), and the present study was aimed to assess the applicability of the ZEF for the human brain histology and immunohistochemistry and to evaluate the detectability of different antigens in the human brain fixed with ZEF. In total, 11 antigens were tested, including NeuN, neuron-specific enolase, GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin, and α-tubulin. The obtained data show that: i) the ZEF has potential for use in general histological practice, where detailed characterization of human brain morphology is needed; ii) the antigens tested are well-preserved in the human brain specimens fixed in the ZEF.

Address: Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine. [email protected].

DOI: 10.4081/ejh.2015.2530

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MeSH Terms: Adult, Aged, Aged, 80 and over, Antigens, Brain, Ethanol, Female, Formaldehyde, Humans, Immunohistochemistry, Male, Middle Aged, Nerve Tissue Proteins, Tissue Fixation, Zinc