Quality control data of physiological and immunological biomarkers measured in serum and plasma.

Eugène Jansen, Piet Beekhof, Johannes Cremers, Birgit Weinberger, Simone Fiegl, Olivier Toussaint, Jürgen Bernhard, Efstathios Gonos, Miriam Capri, Claudio Franceschi, Ewa Sikora, María Moreno-Villanueva, Nicolle Breusing, Tilman Grune, Alexander Bürkle, Martijn E T Dollé

Journal: Mechanisms of ageing and development 2016;151():54-9

PMID: 26166476

Abstract

In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years. The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples. Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values. A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up. Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.

Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

Address: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: [email protected].; Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: [email protected].; Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: [email protected].; Leopold-Franzens-Universität Innsbruck, Institute for Biomedical Aging Research, Rennweg 10, 6020 Innsbruck, Austria. Electronic address: [email protected].; Institute for Nutritional Sciences and Physiology, University for Health Sciences, Medical Informatics and Technology, Eduard Wallnoefer-Zentrum 1, 6060 Hall in Tirol, Austria. Electronic address: [email protected].; Unit of Cellular Biochemistry and Biology, The University of Namur, Rue de Bruxelles 61, 5000 Namur, Belgium. Electronic address: [email protected].; BioTeSys GmbH, Schelztorstraße 54-56, 73,728 Esslingen, Germany. Electronic address: [email protected].; National Hellenic Research Foundation (NHRF) Institute of Biological Research and Biotechnology, 48 Vas. Constantinou Ave., Athens 11635, Greece. Electronic address: [email protected].; DIMES-Department of Experimental, Diagnostic and Specialty Medicine, CIG-Interdepartmentall Centre "L.Galvani", Alma Mater Studiorum, University of Bologna, 40,126 Bologna, Italy. Electronic address: [email protected].; DIMES-Department of Experimental, Diagnostic and Specialty Medicine, CIG-Interdepartmentall Centre "L.Galvani", Alma Mater Studiorum, University of Bologna, 40,126 Bologna, Italy. Electronic address: [email protected].; Nencki Institute of Experimental Biology, Polish Academy of Sciences, St. Pasteura 302-093 Warsaw, Poland. Electronic address: [email protected].; Molecular Toxicology, Department of Biology, Box 628, University of Konstanz, 78,457 Konstanz, Germany. Electronic address: [email protected].; Institute of Nutritional Medicine (180c), University of Hohenheim, Fruwirthstraße 12, 70,599 Stuttgart, Germany. Electronic address: [email protected].; German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Arthur-Scheunert-Allee 114-116, 14,558 Nuthetal, Germany. Electronic address: [email protected].; Molecular Toxicology, Dept of Biology, Box 628, University of Konstanz, 78,457 Konstanz, Germany. Electronic address: [email protected].; Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: [email protected].
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