Modeling and simulation of fructooligosaccharides synthesis in a batch basket reactor.

Maiki Roberto Detofol, Elizama Aguiar-Oliveira, Cindy Elena Bustamante-Vargas, Alexandre Batista de Jesus Soares, Mónica Beatriz Alvarado Soares, Francisco Maugeri

Journal: Journal of biotechnology 2016;210():44-51

PMID: 26130308

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Abstract

Fructooligosaccharides (FOS) production was carried out in a batch basket reactor with immobilized fructosyltransferase from Rhodotorula sp. from 500×10(3) g m(-3) of sucrose in 50 mM sodium acetate buffer at pH 6.0, 48 °C at 85 rpm and with an activity of 22.44×10(3) U m(-3). The experimental data were well adjusted to the mathematical model for FOS production using SIMULINK(®) (MATLAB(®)). The highest regression coefficient (R(2)>90%) and the lowest percentual residual standard deviation (%RSD<4.0) and chi-square (χ(2) <1.0) were obtained for sucrose (GF), kestose (GF2) and total FOS. The mass transfer coefficient (kL) was determined as 5.6×10(-5) m h(-1) and the diffusivity (DS) was 2.11×10(-11) m(2) s(-1). The best predicted FOS yield (after 96 h) was 60.62%, with an equivalent productivity of 3.16×10(3) g m(-3) h(-1). These results reaffirm the good potential of this enzyme for industrial application and, in addition, are in conformation to other studies conducted with the same enzyme from the same and different microbial sources.

Address: Department of Exact and Land Sciences, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil.; Department of Bioscience, Multidisciplinary Institute on Health (IMS), Federal University of Bahia campus Anísio Teixeira (CAT/UFBA), R. Rio de Contas, 80, Candeias, Postal Code: 45.029-094, Vitória da Conquista, Bahia, Brazil. Electronic address: [email protected].; Department of Engineering and Computer Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil.; Department of Agricultural Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99.700-000, Erechim, Rio Grande do Sul, Brazil.; Department of Food Engineer, Faculty of Food Engineer (FEA), University of Campinas (UNICAMP), R. Monteiro Lobato, 80, Cidade Universitária, Postal Code: 13083-862. Campinas, São Paulo, Brazil.

DOI: 10.1016/j.jbiotec.2015.06.410

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MeSH Terms: Bioreactors, Computer Simulation, Enzymes, Immobilized, Fungal Proteins, Hexosyltransferases, Models, Theoretical, Oligosaccharides, Rhodotorula