Effects of nitrogen dioxide and its acid mist on reactive oxygen species production and antioxidant enzyme activity in Arabidopsis plants.

Xiaofang Liu, Fen Hou, Guangke Li, Nan Sang

Journal: Journal of environmental sciences (China) 2016;34():93-9

PMID: 26257351

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Abstract

Nitrogen dioxide (NO2) is one of the most common and harmful air pollutants. To analyze the response of plants to NO2 stress, we investigated the morphological change, reactive oxygen species (ROS) production and antioxidant enzyme activity in Arabidopsis thaliana (Col-0) exposed to 1.7, 4, 8.5, and 18.8 mg/m(3) NO2. The results indicate that NO2 exposure affected plant growth and chlorophyll (Chl) content, and increased oxygen free radical (O2(-)) production rate in Arabidopsis shoots. Furthermore, NO2 elevated the levels of lipid peroxidation and protein oxidation, accompanied by the induction of antioxidant enzyme activities and change of ascorbate (AsA) and glutathione (GSH) contents. Following this, we mimicked nitric acid mist under experimental conditions, and confirmed the antioxidant mechanism of the plant to the stress. Our results imply that NO2 and its acid mist caused pollution risk to plant systems. During the process, increased ROS acted as a signal to induce a defense response, and antioxidant status played an important role in plant protection against NO2/nitric acid mist-caused oxidative damage.

Address: College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan 030006, China.; College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.; College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan 030006, China. Electronic address: [email protected].; College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan 030006, China. Electronic address: [email protected].

DOI: 10.1016/j.jes.2015.03.011

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MeSH Terms: Antioxidants, Arabidopsis, Lipid Peroxidation, Nitric Acid, Nitrogen Dioxide, Oxidation-Reduction, Plant Proteins, Plant Shoots, Reactive Oxygen Species