Effect of sodium accumulation on heterotrophic growth and polyhydroxybutyrate (PHB) production by Cupriavidus necator.

Md Salatul Islam Mozumder, Linsey Garcia-Gonzalez, Heleen De Wever, Eveline I P Volcke

Journal: Bioresource technology 2016;191():213-8

PMID: 25997010

Full Text on Elsevier Science View on PubMed

Abstract

This study evaluates the effect of sodium (Na(+)) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na(+): biomass growth became zero at 8.9 g/L Na(+) concentration while PHB production was completely stopped at 10.5 g/L Na(+). A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na(+) inhibition effect. The parameters related to Na(+) inhibition were estimated based on shake flask experiments. The accumulated Na(+) showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na(+) due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.

Address: Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Boeretang 200, 2400 Mol, Belgium; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Gent, Belgium. Electronic address: [email protected].; Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Boeretang 200, 2400 Mol, Belgium.; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Gent, Belgium.

DOI: 10.1016/j.biortech.2015.04.110

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MeSH Terms: Calibration, Cupriavidus necator, Hydroxybutyrates, Models, Biological, Sodium