Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen.

Sergey A Khorobrykh, Maarit Karonen, Esa Tyystjärvi

Journal: FEBS letters 2015;589(6):779-86

PMID: 25701589

Abstract

Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen ((1)O2) and analyzed the products. The main reaction of (1)O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between (1)O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane.

Address: Department of Biochemistry/Molecular Plant Biology, University of Turku, FI-20014 Turku, Finland.; Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, FI-20014 Turku, Finland.; Department of Biochemistry/Molecular Plant Biology, University of Turku, FI-20014 Turku, Finland. Electronic address: [email protected].

DOI: 10.1016/j.febslet.2015.02.011

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MeSH Terms: Cucurbita, Hydrogen Peroxide, Oxidation-Reduction, Plant Leaves, Plastoquinone, Singlet Oxygen, Thylakoids