The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L.

Marek Kamlar, Olga Rothova, Sarka Salajkova, Dana Tarkowska, Pavel Drasar, Marie Kocova, Juraj Harmatha, Dana Hola, Ladislav Kohout, Tomas Macek

Journal: Steroids 2015;97():107-12

PMID: 25578736

Full Text on Elsevier Science View on PubMed

Abstract

The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.

Address: Dept. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: [email protected].; Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: [email protected].; Dept. of Natural Products, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic; Present address: Biomedical Research Center, University Hospital Hradec Kralove, 500 05 Hradec Kralove, Czech Republic. Electronic address: [email protected].; Laboratory of Growth Regulators, Palacky Univ. and Institute of Experimental Botany ASCR, Olomouc 78371, Czech Republic. Electronic address: [email protected].; Dept. of Natural Products, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: [email protected].; Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: [email protected].; Institute of Organic Chemistry and Biochemistry ASCR, Prague 16610, Czech Republic. Electronic address: [email protected].; Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: [email protected].; Institute of Organic Chemistry and Biochemistry ASCR, Prague 16610, Czech Republic. Electronic address: [email protected].; Dept. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: [email protected].

DOI: 10.1016/j.steroids.2014.12.024

Link outs

Subscription / membership required

Full Text Sources:

MeSH Terms: Brassinosteroids, Ecdysteroids, Molecular Conformation, Plant Leaves, Spinacia oleracea, Steroids, Heterocyclic