Resveratrol induces apoptosis and alters gene expression in human fibrosarcoma cells.

Kamran Harati, Pawel Slodnik, Ansgar Michael Chromik, Ole Goertz, Tobias Hirsch, Nikolai Kapalschinski, Ludger Klein-Hitpass, Jonas Kolbenschlag, Waldemar Uhl, Marcus Lehnhardt, Adrien Daigeler

Journal: Anticancer research 2015;35(2):767-74

PMID: 25667456

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Abstract

BACKGROUND/AIM

Metastatic fibrosarcomas still represent a therapeutic dilemma. Commonly used chemotherapeutic agents such as doxorubicin have been proven effective in fewer than 30% of all cases disseminated of fibrosarcoma. Elderly patients with cardiac disease are not suitable for systemic chemotherapy with doxorubicin. We therefore tested the apoptotic effects of the natural and well-tolerated compound resveratrol on human fibrosarcoma cells (HT1080).

MATERIALS AND METHODS

Vital, apoptotic and necrotic cells were quantified using flow cytometric analysis. Gene expression was analyzed by RNA microarrays.

RESULTS

Application of resveratrol induced apoptotic cell death and significantly reduced proliferation of HT1080 cells. Correspondingly, expression of apoptosis-associated genes was altered in microarray analysis.

CONCLUSION

This in vitro study demonstrates the anticancer activity of resveratrol against human fibrosarcoma cells. These results provide experimental support for in vivo trials assessing the effect of the natural polyphenol resveratrol.

Address: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany [email protected].; Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.; Visceral and General Surgery, St. Josef Hospital Ruhr University Bochum, Bochum, Germany.; Institute for Cell Biology (Tumor Research), University of Duisburg-Essen, Essen, Germany.

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MeSH Terms: Apoptosis, Cell Line, Tumor, Fibrosarcoma, Flow Cytometry, Gene Expression, Humans, Oligonucleotide Array Sequence Analysis, Resveratrol, Stilbenes