Experimental study on apoptosis of HL-60 cell induced by arsenic trioxide.

Z Wang, Y Xu, J Liao

Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 2002;20(7):536-8

PMID: 12043582

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Abstract

OBJECTIVE

To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia.

METHODS

The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed.

RESULTS

After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later.

CONCLUSION

Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

Address: Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Bejing (10091).

Link outs

Free resources

Miscellaneous:

https://assays.cancer.gov/CPTAC-651

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-SERPINA1...

MeSH Terms: Apoptosis, Arsenic Trioxide, Arsenicals, HL-60 Cells, Humans, Oxides, Telomerase