A recombinant PPRE-driven luciferase bioassay for identification of potential PPAR agonists.

Feng-Yuan Tsai, Yi-Ting Cheng, Tsui-Chun Tsou

Journal: Vitamins and hormones 2014;94():427-35

PMID: 24388199

Full Text on Elsevier Science View on PubMed

Abstract

A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.

Address: Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.; Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan. Electronic address: [email protected].

DOI: 10.1016/B978-0-12-800095-3.00015-8

Link outs

Free resources

Other Literature Sources:

https://scite.ai/reports/24388199

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/B978-...

MeSH Terms: Cell Line, Clone Cells, Drug Discovery, Endocrine Disruptors, Genes, Reporter, Green Fluorescent Proteins, High-Throughput Screening Assays, Humans, Luciferases, Peroxisome Proliferator-Activated Receptors, Peroxisome Proliferators, Promoter Regions, Genetic, Recombinant Fusion Proteins, Response Elements, Thymidine Kinase