The amide of galacturonic acid with lysine as an immunodominant component of the lipopolysaccharide core region from Proteus penneri 42 strain.

Agata Palusiak, Anna Maciejewska, Czesław Ługowski, Antoni Różalski

Journal: Acta biochimica Polonica 2014;61(1):129-32

PMID: 24644555

Abstract

Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown the significance of an amide of galacturonic acid with lysine [D-GalA(L-Lys)] for serological specificity of O-antigens from few P. mirabilis strains. In this work, the immunodominant role of GalALys was indicated for the P. penneri 42 LPS core region. The studies also showed the serological identity of core oligosaccharides from P. penneri 42 (O71), P. mirabilis 51/57 (O28) and R14/S1959 strains.

Address: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Łódź, Poland.; Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.; Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland amd Department of Biotechnology and Molecular Biology, University of Opole, Opole, Poland.; Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Łódź, Poland.

MeSH Terms: Amides, Hexuronic Acids, Immunodominant Epitopes, Lipopolysaccharides, Lysine, O Antigens, Proteus penneri