Polycation-mediated integrated cell death processes.

Ladan Parhamifar, Helene Andersen, Linping Wu, Arnaldur Hall, Diana Hudzech, Seyed Moien Moghimi

Journal: Advances in genetics 2015;88():353-98

PMID: 25409612

Full Text on Elsevier Science View on PubMed

Abstract

One of the major challenges in the field of nucleic acid delivery is the design of delivery vehicles with attributes that render them safe as well as efficient in transfection. To this end, polycationic vectors have been intensely investigated with native polyethylenimines (PEIs) being the gold standard. PEIs are highly efficient transfectants, but depending on their architecture and size they induce cytotoxicity through different modes of cell death pathways. Here, we briefly review dynamic and integrated cell death processes and pathways, and discuss considerations in cell death assay design and their interpretation in relation to PEIs and PEI-based engineered vectors, which are also translatable for the design and studying the safety of other transfectants.

Address: Nanomedicine Research Group and Centre for Pharmaceutical Nanotechnology and Nanotoxicology, Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark; NanoScience Centre, University of Copenhagen, Copenhagen Ø, Denmark.; Nanomedicine Research Group and Centre for Pharmaceutical Nanotechnology and Nanotoxicology, Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark.; Centre for BioNano Interactions School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland.; Nanomedicine Research Group and Centre for Pharmaceutical Nanotechnology and Nanotoxicology, Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark; NanoScience Centre, University of Copenhagen, Copenhagen Ø, Denmark; Department of Translation Imaging, Houston Methodist Research Institute, Houston Methodist Hospital Systems, Houston, TX, USA.

DOI: 10.1016/B978-0-12-800148-6.00012-2

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MeSH Terms: Anoikis, Apoptosis, Autophagy, Calcium, Cell Death, Endoplasmic Reticulum Stress, Genetic Vectors, Humans, Lysosomes, Mitochondria, Nanomedicine, Necrosis, Polyamines, Polyelectrolytes, Polyethyleneimine, Transfection