SPION primes THP1 derived M2 macrophages towards M1-like macrophages.

Amit Laskar, Jonas Eilertsen, Wei Li, Xi-Ming Yuan

Journal: Biochemical and biophysical research communications 2014;441(4):737-42

PMID: 24184477

Full Text on Elsevier Science View on PubMed

Abstract

Potentially, cellular iron regulates functional plasticity in macrophages yet; interaction of functionally polarized macrophages with iron-oxide nanoparticles has never been studied. We found that monocyte differentiation alters cellular ferritin and cathepsin L levels and induces functional polarization in macrophages. Iron in super paramagnetic iron-oxide nanoparticle (SPION) induces a phenotypic shift in THP1 derived M2 macrophages towards a high CD86+ and high TNF α+ macrophage subtype. This phenotypic shift was accompanied by up-regulated intracellular levels of ferritin and cathepsin L in M2 macrophages, which is a characteristic hallmark of M1 macrophages. Atherogenic oxysterols reduce phagocytic activity in macrophage subtypes, and thus these cells may escape detection by iron-oxide nanoparticles (INPs) in-vivo.

Address: Experimental Pathology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping 58185, Sweden; Division of Occupational and Environmental Medicine, University Hospital, Linköping 58185, Sweden. Electronic address: [email protected].

DOI: 10.1016/j.bbrc.2013.10.115

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MeSH Terms: B7-2 Antigen, Cathepsin L, Cell Differentiation, Cell Polarity, Cells, Cultured, Dextrans, Ferric Compounds, Ferritins, Humans, Immunoglobulin G, Inflammation, Macrophages, Magnetite Nanoparticles, Phagocytosis, Plaque, Atherosclerotic, Sterols, Tetradecanoylphorbol Acetate, Tumor Necrosis Factor-alpha, Up-Regulation