A yellow fluorescent protein with reduced chloride sensitivity engineered by loop-insertion.

Junyi Liang, Yang Yang, Puguang Yin, Yin Ding, Yan Shen, Meng Qin, Jun Wang, Qiang Xu, Yi Cao, Wei Wang

Journal: Chembiochem : a European journal of chemical biology 2014;14(12):1423-6

PMID: 23868849

Abstract

Light it up! We demonstrate a novel way to reduce the chloride sensitivity of yellow fluorescent protein by inserting glycine residues in its loop region. The length and position for the insertion were optimized experimentally, and a plausible underlying mechanism is proposed.

Address: National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Jiangsu 210008, China.

DOI: 10.1002/cbic.201300199

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MeSH Terms: Bacterial Proteins, Chlorides, Glycine, Luminescent Proteins, Mutagenesis, Insertional, Protein Engineering