Augmented platelet calcium uptake in response to serotonin stimulation in patients with major depression measured using Mn2+ influx and 45Ca2+ uptake.

H Plein, M Berk, S Eppel, N Butkow

Journal: Life sciences 2000;66(5):425-31

PMID: 10670830

Full Text on Elsevier Science View on PubMed

Abstract

There is an augmented platelet intracellular calcium response to serotonin stimulation in major depression. The role that calcium influx has in this process is not known. The objective of this study was to determine platelet calcium influx in response to serotonin by two methods, Mn2+ influx and 45Ca2+ uptake, in order to observe if the uptake response to serotonin was augmented in major depression by comparing the response to normal controls. The use of the two methods of calcium influx showed that serotonin stimulates calcium uptake into platelets. Furthermore, patients with major depression have significantly augmented platelet calcium uptake in response to serotonin. The interesting finding was that calcium uptake into platelets is biphasic, occurring immediately and after five minutes. These results may support the two pool model for calcium oscillations within cells whereby extracellular calcium is needed for intracellular calcium release, and for replenishment of depleted stores once intracellular calcium is released.

Address: Department of Clinical and Experimental Pharmacology, University of the Witwatersrand, Faculty of Health Sciences, Parktown, Johannesburg.

DOI: 10.1016/s0024-3205(99)00608-6

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0024...

MeSH Terms: Adult, Aged, Blood Platelets, Calcium, Calcium Radioisotopes, Cations, Divalent, Depressive Disorder, Dose-Response Relationship, Drug, Female, Fluorescence, Fura-2, Humans, Ion Transport, Kinetics, Male, Manganese, Middle Aged, Serotonin