West Nile virus protease activity in detergent solutions and application for affinity tag removal.

Qiwei Huang, Qingxin Li, Angela Shuyi Chen, Congbao Kang

Journal: Analytical biochemistry 2013;435(1):44-6

PMID: 23291011

Full Text on Elsevier Science View on PubMed

Abstract

Affinity tags are typically used to facilitate membrane protein purification. A protease needs to remain active in the presence of detergent to remove a fusion tag from a recombinant membrane protein. Our results show that the West Nile virus (WNV) protease activity was not affected while in the presence of a wide range of detergents. In a detergent solution, the WNV protease can remove the fusion tag from a recombinant protein containing KCNE3 and a WNV protease site. Therefore, the WNV protease may be useful as an alternative enzyme to remove affinity tags in protein purifications.

Address: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A∗STAR), Singapore 138669, Singapore.

DOI: 10.1016/j.ab.2012.12.015

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MeSH Terms: Amino Acid Sequence, Chromatography, Affinity, Detergents, Escherichia coli, Humans, Molecular Sequence Data, Peptide Hydrolases, Peptides, Potassium Channels, Voltage-Gated, Recombinant Proteins, Solutions, West Nile virus