A novel statistical analysis and interpretation of flow cytometry data.

H T Banks, D F Kapraun, W Clayton Thompson, Cristina Peligero, Jordi Argilaguet, Andreas Meyerhans

Journal: Journal of biological dynamics 2013;7(1):96-132

PMID: 23826744

Abstract

A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4+T and CD8+T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labelled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide.

Address: Center for Research in Scientific Computation and Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC 27695-8212, USA. [email protected]

DOI: 10.1080/17513758.2013.812753

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https://scite.ai/reports/23826744

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https://antibodies.cancer.gov/detail/CPTC-CD4-1

MeSH Terms: CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Proliferation, Data Interpretation, Statistical, Flow Cytometry, Fluoresceins, Fluorescent Dyes, Humans, Mathematical Concepts, Mitogens, Models, Immunological, Models, Statistical, Phytohemagglutinins, Succinimides