Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers.

Chao Liu, Jingwu Kang

Journal: Journal of chromatography. A 2012;1238():146-51

PMID: 22472476

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Abstract

An improved CE-FA method using a polyelectrolyte multilayer (PEML) coated capillary is described. The coating was simply created by alternatively flushing the capillary with positively charged polyelectrolyte polybrene (HDB) and negatively charged polyelectrolyte dextran sulfate (DS). Human serum albumin (HSA) and 6 drugs were selected as an experimental model to evaluate such an improvement. The PEML coating was demonstrated to effectively reduce the protein adsorption on the capillary wall. Compared with the uncoated capillary, the repeatability (RSD%) of the EOF mobility and the plateau height of free drug were improved significantly from 3.7% to 0.51% and from 0.61% to 0.34%, respectively. No interference of the coating on the measurement of protein-drug binding parameters was observed.

Address: Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Lingling Road 345, Shanghai 200032, China.

DOI: 10.1016/j.chroma.2012.03.043

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MeSH Terms: Adsorption, Dextran Sulfate, Electrolytes, Electrophoresis, Capillary, Hexadimethrine Bromide, Humans, Models, Chemical, Nonlinear Dynamics, Pharmaceutical Preparations, Reproducibility of Results, Serum Albumin