Neonatal red blood cells: amiloride-insensitive Na+-H+ transport isoform would express Na+-Li+ exchange.

R E Serrani, G Mujica, I A Gioia, J L Corchs

Journal: Acta physiologica et pharmacologica Bulgarica 2001;25(3-4):71-4

PMID: 11688549

Abstract

Neonatal red cells (umbilical cord blood) were in vitro incubated in isotonic media (thiocyanate as predominant anion). This experimental condition was selected as it was known that this chaotropic anion induced activation of Na+-H+ exchange. The transport amiloride-sensitive mechanism, that decreased the acidic intracellular pH change occurring in this medium, would correspond to Na+-H+ exchange (NHE1 isoform). However, the Na+-Li+ exchange, also determined in the cells in the above-mentioned medium was not affected by amiloride. The present data suggest that an amiloride insensitive Na+-H+ exchange isoform would express Na+-Li+ countertransport in these cells.

Address: Department of Physiology, School of Medicine, National University of Rosario, Santa Fe, Argentina.

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MeSH Terms: Amiloride, Cations, Monovalent, Cells, Cultured, Erythrocytes, Humans, Infant, Newborn, Ion Transport, Lithium, Protein Isoforms, Sodium, Sodium-Hydrogen Exchangers, Time Factors