Isolation of a strawberry gene fragment encoding an actin depolymerizing factor-like protein from genotypes resistant to Colletotrichum acutatum.

Marta Ontivero, Gustavo Martínez Zamora, Sergio Salazar, Juan Carlos Díaz Ricci, Atilio Pedro Castagnaro

Journal: Genome 2012;54(12):1041-4

PMID: 22107362

Abstract

Actin depolymerizing factors (ADFs) have been recently implicated in plant defense against pathogenic fungi, associated with the cytoskeletal rearrangements that contribute to establish an effective barrier against fungal ingress. In this work, we identified a DNA fragment corresponding to a part of a gene predicted to encode an ADF-like protein in genotypes of Fragaria ananassa resistant to the fungus Colletotrichum acutatum. Bulked segregant analysis combined with AFLP was used to identify polymorphisms linked to resistance in hybrids derived from the cross between the resistant cultivar 'Sweet Charlie' and the susceptible cultivar 'Pájaro'. The sequence of one out of three polymorphic bands detected showed significant BLASTX hits to ADF proteins from other plants. Two possible exons were identified and bioinformatic analysis revealed the presence of the ADF homology domain with two actin-binding sites, an N-terminal phosphorylation site, and a nuclear localization signal. In addition to its possible application in strawberry breeding programs, these finding may contribute to investigate the role of ADFs in plant resistance against fungi.

Address: Sección Biotecnología, Estación Experimental Agroindustrial Obispo Colombres-Unidad Asociada al INSIBIO, Av. William Cross 3150, 4101 Las Talitas, Tucumán, Argentina.

DOI: 10.1139/g11-068

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MeSH Terms: Actin Depolymerizing Factors, Amino Acid Sequence, Amplified Fragment Length Polymorphism Analysis, Binding Sites, Chimera, Colletotrichum, Computational Biology, Crosses, Genetic, DNA, Plant, Exons, Fragaria, Genes, Plant, Genotype, Molecular Sequence Data, Nuclear Localization Signals, Phosphorylation, Plant Diseases, Polymorphism, Genetic, Protein Structure, Tertiary, Sequence Alignment