Crystal structure of human germline antibody 3-23/B3.

Thomas J Malia, Galina Obmolova, Juan Carlos Almagro, Gary L Gilliland, Alexey Teplyakov

Journal: Molecular immunology 2011;48(12-13):1586-8

PMID: 21605907

Full Text on Elsevier Science View on PubMed

Abstract

The human antibody repertoire is dominated by a few combinations of germline sequences, with the genes 3-23 for the heavy chain and B3 for the light chain being among the most frequently used. Despite this fact, there was no experimental structure of the antibody composed of 3-23 and B3. The crystal structure of the Fab fragment of the synthetic antibody composed of the 3-23 and B3 germline sequences was determined to provide a template for antibody modeling. The antigen-binding loops were found in the canonical conformations. Comparison to the other structures where either 3-23 or B3 is paired with a different chain reveals a significant deviation in the orientation of the variable domains.

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DOI: 10.1016/j.molimm.2011.04.020

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MeSH Terms: Amino Acid Sequence, Antibodies, Antigen-Antibody Complex, Binding Sites, Antibody, Crystallography, X-Ray, Humans, Immunoglobulin Fab Fragments, Models, Molecular, Protein Conformation