BPA uptake does not correlate with LAT1 and Ki67 expressions in tumor samples (results of EORTC trial 11001).

Andrea Wittig, Sien-Yi Sheu-Grabellus, Laurence Collette, Raymond Moss, Lorenzo Brualla, Wolfgang Sauerwein

Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2012;69(12):1807-12

PMID: 21367608

Full Text on Elsevier Science View on PubMed

Abstract

The system L-amino acid transporter (LAT1) imports p-boronophenylalanine (BPA) mainly into proliferating cells. This study investigates in three tumor entities whether the proportion of tumor cells expressing LAT1 and/or Ki67 correlates with BPA uptake. Tumors were analyzed for (10)B concentration with prompt gamma-ray spectroscopy and for Ki67 and LAT1 expressions with immunohistochemical methods. The proportion of LAT1-expressing cells was much higher (5-90%) than that of Ki67-expressing cells (0-20%) and cells expressing both Ki67 and LAT1 (0-5%). Neither LAT1 nor Ki67 expression predicted BPA uptake.

Address: Department of Radiotherapy and Radiation Oncology, University Hospital Marburg, Philipps-University Marburg, Marburg, Germany. [email protected]

DOI: 10.1016/j.apradiso.2011.02.018

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MeSH Terms: Boron Compounds, Humans, Immunohistochemistry, Ki-67 Antigen, Large Neutral Amino Acid-Transporter 1, Phenylalanine, Spectrometry, Gamma