Expanding and engineering the genetic code in a single expression experiment.

Nediljko Budisa, Michael G Hoesl

Journal: Chembiochem : a European journal of chemical biology 2012;12(4):552-5

PMID: 22238148

Abstract

Expanding and engineering the code simultaneously: This concept was experimentally realized in a single in vivo expression experiment whereby residue-specific, sense codon reassignments Met→Nle/Pro→(4S-F)Pro (code engineering) were combined with position-specific STOP→Bpa read-through by an amber suppressor tRNA (code expansion).

Address: Max Planck Institute of Biochemistry, Molecular Biotechnology, Am Klopferspitz 18, 82152 Martinsried, Germany.

DOI: 10.1002/cbic.201000586

Link outs

Subscription / membership required

Full Text Sources:

https://doi.org/10.1002/cbic.201000586

Other Literature Sources:

https://facultyopinions.com/pubmed/22238148

MeSH Terms: Amino Acids, Amino Acyl-tRNA Synthetases, Gene Expression Profiling, Genetic Code, Molecular Structure, Protein Engineering, Recombinant Proteins, Suppression, Genetic