The endocytic penetration mechanism of iron oxide magnetic nanoparticles with positively charged cover: a morphological approach.

Magdalena Cañete, Jorge Soriano, Angeles Villanueva, Alejandro G Roca, Sabino Veintemillas, Carlos J Serna, Rodolfo Miranda, Maria Del Puerto Morales

Journal: International journal of molecular medicine 2011;26(4):533-9

PMID: 20818493

Full Text on Ingenta plc View on PubMed

Abstract

In this study we present a morphological approach in observing the interaction of cationic magnetic nanoparticles with A-549 cells (human lung adenocarcinoma). Under our experimental conditions, nanoparticles easily penetrated cells and were observed in vivo, using bright light microscopy. In fixed cells, nanoparticles remained inside cells, showing quantity and distribution patterns similar to those in unfixed cells. The presence of nanoparticles did not affect cell viability or the morphologic parameters assessed. We determined the potential internalization mechanism of nanoparticles into cells using endocytosis inhibitors. The results suggest that nanoparticles used in this study penetrate A-549 cells mainly through a macropinocytosis process.

Address: Departamento de Biología, Universidad Autónoma de Madrid C/ Darwin 2, Cantoblanco, Madrid, Spain. [email protected]

DOI: 10.3892/ijmm_00000496

Link outs

Subscription / membership required

Full Text Sources:

MeSH Terms: Cations, Cell Line, Tumor, Cell Survival, Clathrin, Endocytosis, Ferric Compounds, Humans, Magnetics, Microtubules, Nanoparticles, Particle Size, Static Electricity, Tubulin