A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein.

Debin Zhu, Xiaoming Zhou, Da Xing

Journal: Biosensors & bioelectronics 2011;26(1):285-8

PMID: 20637592

Full Text on Elsevier Science View on PubMed

Abstract

A new kind of aptamer-based immunomagnetic electrochemiluminescence (IM-ECL) assay for quantitative detection of protein is developed. The assay consists of a double aptamer sandwich format in which a biotin-labeled aptamer is used for rapid and specific capture of target protein, and a [Ru(bpy)(3)](2+) (TBR)-labeled aptamer is used for ECL detection. As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method. Experimental results show that the detection limit of the assay is 80 pmol/L of PDGF-BB. A calibration curve with a linearity range from 0.1 to 1000 nmol/L is established, thus, make quantitative analysis possible. The method has been used to detect PDGF-BB in fetal calf serum with minimum background interference. Due to the wide availability of aptamer for numerous proteins, this new method holds great promise in protein detection.

Address: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

DOI: 10.1016/j.bios.2010.06.028

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MeSH Terms: Aptamers, Peptide, Biosensing Techniques, Conductometry, Equipment Design, Equipment Failure Analysis, Immunomagnetic Separation, Luminescent Measurements, Proteins