Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake.

Xiang Ding, Jie Tang, Mei Cao, Chun-Xiao Guo, Xia Zhang, Jing Zhong, Jie Zhang, Qun Sun, Su Feng, Zhi-Rong Yang, Jian Zhao

Journal: International journal of biological macromolecules 2010;47(2):271-5

PMID: 20430053

Full Text on Elsevier Science View on PubMed

Abstract

In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-beta-d-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an (1-->3)-alpha-d-galactopyranose residue, and terminated with alpha-d-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH(-) radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide.

Address: Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, PR China.

DOI: 10.1016/j.ijbiomac.2010.04.010

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MeSH Terms: Biphenyl Compounds, Carbohydrate Sequence, Chromatography, Free Radical Scavengers, Fruiting Bodies, Fungal, Hydroxyl Radical, Molecular Sequence Data, Picrates, Polysaccharides, Solubility, Superoxides, Tricholoma, Water