Oxidative stress-dependent p66Shc phosphorylation in skin fibroblasts of children with mitochondrial disorders.

Magdalena Lebiedzinska, Agnieszka Karkucinska-Wieckowska, Carlotta Giorgi, Elzbieta Karczmarewicz, Ewa Pronicka, Paolo Pinton, Jerzy Duszynski, Maciej Pronicki, Mariusz R Wieckowski

Journal: Biochimica et biophysica acta 2011;1797(6-7):952-60

PMID: 20226758

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Abstract

p66Shc, the growth factor adaptor protein, can have a substantial impact on mitochondrial metabolism through regulation of cellular response to oxidative stress. We investigated relationships between the extent of p66Shc phosphorylation at Ser36, mitochondrial dysfunctions and an antioxidant defense reactions in fibroblasts derived from five patients with various mitochondrial disorders (two with mitochondrial DNA mutations and three with methylglutaconic aciduria and genetic defects localized, most probably, in nuclear genes). We found that in all these fibroblasts, the extent of p66Shc phosphorylation at Ser36 was significantly increased. This correlated with a substantially decreased level of mitochondrial superoxide dismutase (SOD2) in these cells. This suggest that SOD2 is under control of the Ser36 phosphorylation status of p66Shc protein. As a consequence, an intracellular oxidative stress and accumulation of damages caused by oxygen free radicals are observed in the cells.

Address: Nencki Institute of Experimental Biology, Warsaw, Poland.

DOI: 10.1016/j.bbabio.2010.03.005