Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis.

Antonio Jesús Castro, Audrey Bednarczyk, Christine Schaeffer-Reiss, María Isabel Rodríguez-García, Alain Van Dorsselaer, Juan de Dios Alché

Journal: Proteomics 2010;10(5):953-62

PMID: 20049857

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Abstract

In the present paper, we have used 2-DE coupled to MS analysis to examine the molecular variability of the Ole e 1 allergen in three olive cultivars (cvs). Our results confirmed that the predicted polymorphism of Ole e 1 at cDNA level is extended to the expressed protein. The profiles of both the Ole e 1 peptides and the N-glycan variants significantly changed among cvs. We observed that Picual and Arbequina cvs presented the highest and lowest degree of Ole e 1 polymorphism, respectively. Some of these peptides and N-glycans were distributed in a cv-specific manner. The putative implications of this molecular polymorphism in the development of the allergy symptoms are discussed.

Address: Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain. [email protected]

DOI: 10.1002/pmic.200900192

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MeSH Terms: Allergens, Amino Acid Sequence, Antibodies, Antigens, Plant, Chromatography, Liquid, Densitometry, Electrophoresis, Gel, Two-Dimensional, Glycopeptides, Mass Spectrometry, Molecular Sequence Data, Olea, Peptide Mapping, Peptides, Plant Extracts, Plant Proteins, Polymorphism, Genetic, Polysaccharides, Proteome, Silver Staining