Overview of the synthesis of nucleoside phosphates and polyphosphates.

David C Johnson, Theodore S Widlanski

Journal: Current protocols in nucleic acid chemistry 2008;Chapter 13():Unit 13.1

PMID: 18428921

Abstract

This overview summarizes methodology used for the synthesis of nucleoside mono-, di-, and triphosphates. Selected techniques such as the Mitsunobu reaction, displacement reactions involving nucleoside 5'-tosylates, "anion-exchange" techniques, and phosphoramidite and phosphoramidate methodologies are highlighted. The chemistry of phosphorylation is detailed with respect to advantages and limitations under various conditions. Applicability of the methods toward the synthesis of analogs such as imidophosphates, phosphorothioates, and radiolabeled nucleotides is also addressed.

Address: Indiana University, Bloomington, Indiana, USA.

DOI: 10.1002/0471142700.nc1301s15

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https://doi.org/10.1002/0471142700.nc1301s15

MeSH Terms: Amides, Anhydrides, Catalysis, Models, Biological, Nucleosides, Nucleotides, Oligonucleotides, Organophosphonates, Phosphates, Phosphoadenosine Phosphosulfate, Phosphoric Acids, Phosphorus, Phosphorylation, Polyphosphates