Engineering terminal disulfide bonds into DNA.

Gary D Glick

Journal: Current protocols in nucleic acid chemistry 2008;Chapter 5():Unit 5.7

PMID: 18428912

Abstract

This protocol presents a simple and general means of modifying nucleic acids with disulfide cross-links. These cross-links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of DNA and RNA, much in the way that cystine cross-links have been used to study proteins. The chemistry described has been used to synthesize disulfide-cross-linked hairpins and duplexes, higher-order structures such as triplexes, non-ground-state conformations, and tRNAs. since the cross-links form quantitatively by mild air oxidation and do not purturb either secondary or tertiary structure, this modification should prove quite useful for the study of nucleic acids.

Address: University of Michigan, Ann Arbor, Michigan, USA.

DOI: 10.1002/0471142700.nc0507s13

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https://doi.org/10.1002/0471142700.nc0507s13

MeSH Terms: Base Sequence, Cross-Linking Reagents, DNA, DNA Probes, Disulfides, Genetic Engineering, Models, Biological, Molecular Sequence Data, Nucleic Acid Conformation, Nucleic Acid Denaturation, Nucleic Acid Heteroduplexes, Nucleosides, Research Design, Thermodynamics