ATP-sensitive cation-channel in wheat (Triticum durum Desf.): identification and characterization of a plant mitochondrial channel by patch-clamp.

Umberto De Marchi, Vanessa Checchetto, Manuela Zanetti, Enrico Teardo, Mario Soccio, Elide Formentin, Giorgio Mario Giacometti, Donato Pastore, Mario Zoratti, Ildikò Szabò

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2011;26(6):975-82

PMID: 21220928

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Abstract

Indirect evidence points to the presence of K(+) channels in plant mitochondria. In the present study, we report the results of the first patch clamp experiments on plant mitochondria. Single-channel recordings in 150 mM potassium gluconate have allowed the biophysical characterization of a channel with a conductance of 150 pS in the inner mitochondrial membrane of mitoplasts obtained from wheat (Triticum durum Desf.). The channel displayed sharp voltage sensitivity, permeability to potassium and cation selectivity. ATP in the mM concentration range completely abolished the activity. We discuss the possible molecular identity of the channel and its possible role in the defence mechanisms against oxidative stress in plants.

Address: Department of Experimental Biomedical Sciences, CNR Institute of Neuroscience, University of Padova, Padova, Italy. [email protected]

DOI: 10.1159/000324010

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MeSH Terms: Adenosine Triphosphate, Mitochondria, Oxidative Stress, Patch-Clamp Techniques, Potassium Channels, Triticum