Expression artifact with retroviral vectors based on pBMN.

Yoshitaka Fukunaga, Robert A Svoboda, Ronald L Cerny, Keith R Johnson, Margaret J Wheelock

Journal: Analytical biochemistry 2009;395(1):49-53

PMID: 19602420

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Abstract

While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.

Address: Department of Oral Biology, University of Nebraska Medical Center, Omaha, 68198, USA.

DOI: 10.1016/j.ab.2009.07.014

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MeSH Terms: Alternative Splicing, Amino Acid Sequence, Artifacts, Catenins, Cell Adhesion Molecules, Cell Line, Codon, DNA, Complementary, Exons, Gene Expression, Genetic Vectors, Humans, Molecular Sequence Data, Moloney murine leukemia virus, Phosphoproteins, Polyproteins, Protein Isoforms, Recombinant Fusion Proteins, Transfection, Delta Catenin