Development of a Method for Isolation of Mature Cardiomyocytes from Human Heart Biopsy Specimens.

S G Kovalenko, Sh R Frolova, V K Kramkova, A K Berezovskii, M A Popov, D V Shumakov, D I Zybin, E G Agafonov, V V Dontsov, K I Agladze

Journal: Bulletin of experimental biology and medicine 2023;175(4):585-591

PMID: 37768452

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Abstract

To increase the yield of living cells and their survival, studies were carried out to optimize the method for isolating cardiomyocytes from biopsy specimens excised from the right atrial appendages. It was found that creatine, blebbistatin, and taurine are necessary components of the buffer solution during cardiomyocyte isolation, and that composition of the solutions is a more important factor than their oxygenation.

Address: Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia. [email protected].; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia. [email protected].

DOI: 10.1007/s10517-023-05907-x

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https://dx.doi.org/10.1007/s10517-023-05907-x

MeSH Terms: Humans, Myocytes, Cardiac, Cell Separation, Taurine