Engineering functional homopolymeric amino acids: from biosynthesis to design.

Yibin Qiu, Delei Xu, Peng Lei, Sha Li, Hong Xu

Journal: Trends in biotechnology 2024;42(3):310-325

PMID: 37775417

Full Text on Elsevier Science View on PubMed

Abstract

Homopolymeric amino acids (HPAs) are a class of microbial polymers that can be classified into two categories: anionic and cationic HPAs. Notable examples include γ-poly-glutamic acid (γ-PGA) and ε-poly-L-lysine (ε-PL) that have wide-ranging applications in medicine, food, and agriculture. The primary method of manufacture is through microbial synthesis. In recent decades significant efforts have been made to enhance the production of HPAs, specifically focusing on γ-PGA and ε-PL. We comprehensively review current advances in understanding the synthetic mechanisms as well as metabolic engineering and fermentation process techniques to improve the production of HPAs. In addition, we discuss the major challenges and solutions associated with desired structure regulation of HPAs and the development of novel structures.

Address: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China.; College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, PR China.; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: [email protected].; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China; Nanjing Shineking Biotech Co. Ltd., Nanjing 210061, PR China. Electronic address: [email protected].

DOI: 10.1016/j.tibtech.2023.08.010

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MeSH Terms: Amino Acids, Fermentation, Metabolic Engineering