Preparation of CLA ascorbyl ester with improved volumetric productivity by an ionic liquid-based reaction system.

Bilian Chen, Zheng Guo, Mette Bruni Let, Bena-Marie Lue, Xuebing Xu

Journal: Organic & biomolecular chemistry 2008;6(17):3196-201

PMID: 18698480

Full Text on Royal Society of Chemistry View on PubMed

Abstract

A new approach to the enzymatic production of conjugated linoleic acid (CLA) ascorbyl ester with a remarkably high volumetric productivity (120-200 g L(-1)) has been developed, in which strong solvation by tOMA.TFA (methyltrioctylammonium trifluoroacetate) enables a high concentration of ascorbic acid to be applied, and in which t-butanol enhances conversion by changing the equilibrium constant of the activity coefficients. This work has experimentally demonstrated the practicability of achieving efficient reactions of polar compounds at high concentrations in ionic liquids without significant loss of enzyme activity.

Address: National Food Institute, Technical University of Denmark, Building 227, DK-2800 KgsLyngby, Denmark.

DOI: 10.1039/b807710j

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MeSH Terms: Ascorbic Acid, Bioreactors, Candida, Chromatography, High Pressure Liquid, Enzymes, Immobilized, Esterification, Esters, Fungal Proteins, Imidazoles, Ionic Liquids, Linoleic Acids, Conjugated, Lipase, Quaternary Ammonium Compounds, Temperature, Thermodynamics, Trifluoroacetic Acid, tert-Butyl Alcohol