Optimization of cold-active protease production by the psychrophilic bacterium Colwellia sp. NJ341 with response surface methodology.

Quanfu Wang, Yanhua Hou, Zhong Xu, Jinlai Miao, Guangyou Li

Journal: Bioresource technology 2008;99(6):1926-31

PMID: 17499500

Full Text on Elsevier Science View on PubMed

Abstract

Culture conditions were optimized for an extracellular cold-active protease production by the psychrophilic bacterium Colwellia sp. NJ341. Response surface methodology was applied for the most significant fermentation parameters (casein, citrate sodium, temperature and Tween-80) identified earlier by one-factor-at-a-time approach. A 2(4) full factorial central composite design was employed to determine the maximum protease production. Using this methodology, the quadratic regression model of producing cold-active protease was built and the optimal combinations of media constituents for maximum protease production (183.21 U/mL) were determined as casein 5.18 g/L, citrate sodium 3.84 g/L, temperature 7.96 degrees C, Tween-80 0.23 g/L. Protease production obtained experimentally coincident with the predicted value and the model was proven to be adequate.

Address: Harbin Institute of Technology, School of the Ocean, Weihai, PR China.

DOI: 10.1016/j.biortech.2007.03.028

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MeSH Terms: Alteromonadaceae, Analysis of Variance, Biotechnology, Cold Temperature, Culture Media, Endopeptidases, Enzyme Stability, Fermentation, Models, Statistical, Peptide Hydrolases, Regression Analysis, Reproducibility of Results, Surface Properties, Temperature, Time Factors