Expression of AIRE in thymocytes and peripheral lymphocytes.

Eriko Suzuki, Yoshihiro Kobayashi, Osamu Kawano, Katsuhiko Endo, Hiroshi Haneda, Haruhiro Yukiue, Hidefumi Sasaki, Motoki Yano, Masanobu Maeda, Yoshitaka Fujii

Journal: Autoimmunity 2008;41(2):133-9

PMID: 18324482

Free Full Text on Atypon View on PubMed

Abstract

Autoimmune regulator (AIRE) is a transcription factor expressed in the thymic medullary epithelial cells (MECs). MECs have been suggested to contribute to tolerance induction by presenting tissue specific antigens to T cells that react to these antigens. AIRE expression in cells other than MECs has not been studied extensively. Here we report four-color flow cytometric and quantitative real-time PCR studies on the expression of AIRE in the lymphoid cells in the thymus and periphery. In the thymus AIRE was expressed in B cells and CD4+CD8+ double positive cells at a low level. In the peripheral blood AIRE was expressed in B cells but not in T cells, and also expressed in CD14+ dendritic cells (DCs)/macrophages and granulocytes at higher levels. AIRE mRNA was expressed higher in B cells than in T cells. Further study is warranted to define the functional role of the AIRE expressed in the lymphoid cells.

Address: Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan.

DOI: 10.1080/08916930701773941

Link outs

Free resources

Full Text Sources:

https://www.tandfonline.com/doi/10.1080/0891693070...

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-CD14-1

MeSH Terms: Adult, Amino Acid Sequence, B-Lymphocytes, Child, Child, Preschool, Dendritic Cells, Flow Cytometry, Humans, Infant, Infant, Newborn, Lipopolysaccharide Receptors, Macrophages, Molecular Sequence Data, Polymerase Chain Reaction, T-Lymphocytes, Thymus Gland, Transcription Factors, AIRE Protein