Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures.

Michel de Vries, Krzysztof Pyrc, Ron Berkhout, Wilma Vermeulen-Oost, Ronald Dijkman, Maarten F Jebbink, Sylvia Bruisten, Ben Berkhout, Lia van der Hoek

Journal: Journal of clinical microbiology 2008;46(2):759-62

PMID: 18077635

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Abstract

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.

Address: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Amsterdam, The Netherlands.

DOI: 10.1128/JCM.02009-07

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MeSH Terms: Amino Acid Sequence, Humans, Molecular Sequence Data, Neutralization Tests, Parechovirus, Picornaviridae, Picornaviridae Infections, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Viral Proteins, Virus Cultivation