Autofluorescent proteins.

Ian M Dobbie, Noel F Lowndes, Kevin F Sullivan

Journal: Methods in cell biology 2008;85():1-22

PMID: 18155456

Full Text on Elsevier Science View on PubMed

Abstract

Autofluorescent proteins (AFPs) have revolutionized molecular cell biology, and applications continue to harness the power of these genetically encoded fluorescent tags. Here, we review the discovery and physical properties of AFPs as well as their development through mutational optimization for several functional parameters. A practical guide to selection and use of major AFPs is provided as well as an overview of techniques for experimental applications.

Address: Department of Biochemistry, National University of Ireland, Galway, Ireland.

DOI: 10.1016/S0091-679X(08)85001-7

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MeSH Terms: Amino Acid Sequence, Fluorescence Recovery After Photobleaching, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Green Fluorescent Proteins, Luminescent Proteins, Molecular Sequence Data, Protein Conformation, Proteins, Recombinant Fusion Proteins, Sequence Alignment