Nanoelectroporation: a first look.

Raji Sundararajan

Journal: Methods in molecular biology (Clifton, N.J.) 2008;423():109-28

PMID: 18370193

Abstract

As the medical field moves from treatment of diseases with drugs to treatment with genes, safe and efficient gene delivery systems are needed to make this transition. One such safe, nonviral, and efficient gene delivery system is electroporation (electrogenetherapy). Exciting discoveries by using electroporation could make this technique applicable to drug and vaccine delivery in addition to gene delivery. Typically, milli- and microsecond pulses have been used for electroporation. Recently, the use of nanosecond electric pulses (10-300 ns) at very high magnitudes (10-300 kV/cm) has been studied for direct DNA transfer to the nucleus in vitro. This article reviews the work done using high intensity, nanopulses, termed as nanoelectroporation (nano-EP), in electroporation gene delivery systems.

Address: ECET Department, Knoy Hall of Technology, West Lafay-ette, IN, USA.

DOI: 10.1007/978-1-59745-194-9_7

Link outs

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Medical:

https://medlineplus.gov/genesandgenetherapy.html

Miscellaneous:

https://assays.cancer.gov/CPTAC-76

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-HLA-B-1

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Full Text Sources:

https://doi.org/10.1007/978-1-59745-194-9_7

MeSH Terms: Apoptosis, Calcium Signaling, Caspases, Cell Line, Cytochromes c, DNA, Recombinant, Electrochemotherapy, Electroporation, Equipment Design, Genetic Therapy, Humans, Nanotechnology, Neoplasms, Phosphatidylserines, Sodium