The first examples of mRNA cap analogs bearing boranophosphate modification.

Joanna Kowalska, Joanna Zuberek, Zbigniew M Darzynkiewicz, Maciej Lukaszewicz, Edward Darzynkiewicz, Jacek Jemielity

Journal: Nucleic acids symposium series (2004) 2010

PMID: 18776367

Abstract

The syntheses of mRNA cap analogs modified with boranophosphate moiety at either the alpha or beta-position of the 5', 5'-triphosphate bridge (m(7)Gppp(BH3)G, m(7)Gpp(BH3)pG and m(7)Gpp(BH3)pm(7)G) are described. The preliminary biological characterization of these compounds revealed that they have high affinity for translational factor eIF4E and high potency to inhibit cap-dependent translation in cell free system. The analogs modified at the beta-position were also found to be resistant to DcpS decapping pyrophosphatase.

Address: Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.

DOI: 10.1093/nass/nrn146

Link outs

Free resources

Other Literature Sources:

https://www.lens.org/lens/search/patent/list?q=ref...

Miscellaneous:

https://assays.cancer.gov/CPTAC-325

MeSH Terms: Boranes, Eukaryotic Initiation Factor-4E, Phosphates, Protein Biosynthesis, RNA Cap Analogs