Examining Ca2+ extrusion of Na+/Ca2+-K+ exchangers.

Haider F Altimimi, Paul P M Schnetkamp

Journal: Annals of the New York Academy of Sciences 2007;1099():29-33

PMID: 17303830

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Abstract

Na+/Ca2+-K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca2+ extrusion) mode of exchange of NCKX2.

Address: Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, 3330 Hospital Dr., N.W. Calgary, Alberta, T2N 4N1, Canada.

DOI: 10.1196/annals.1387.056

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MeSH Terms: Calcium, Cell Line, Electrochemistry, Humans, Sodium-Calcium Exchanger