Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress.

S R McAnulty, P A Hosick, L S McAnulty, J C Quindry, L Still, M B Hudson, A N Dibarnardi, G L Milne, J D Morrow, M D Austin

Journal: Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2008;32(6):1148-55

PMID: 18059589

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Abstract

Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at approximately 80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a double-blind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group)x2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey-Kramer multiple comparison tests, and Student's t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p

Address: Department of Health, Leisure, and Exercise Science, Appalachian State University, Boone, NC 28608, USA. [email protected]

DOI: 10.1139/H07-131

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MeSH Terms: Adolescent, Adult, Anaerobic Threshold, Antioxidants, Ascorbic Acid, Body Composition, Body Weight, Exercise, Ferric Compounds, Humans, Isoprostanes, Lactic Acid, Lipid Peroxidation, Male, Nitrates, Nitrites, Oxidation-Reduction, Oxidative Stress, Probenecid, Uric Acid, Uricosuric Agents