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[Saliva diagnostic in the research about human immune adaptation to study stress and to differrent water drinking behavior.].
Melnik, KN, Baisheva, GM, Gilmiyarova, FN, Alpatova, TA
Klinicheskaia laboratornaia diagnostika. 2018;(6):353-357
Abstract
85 healthy young people were participates of a randomized placebo controlled cross-over fashion. This study tested associations between different water drinking behavior, the condition of oral immune protection and stress factors over 3 months. We examined saliva IL-1, IL-4, IL-6, TNF-α, γ-IFN, α-amylase and compared them with stress-associated psychophysiological data. As a result of our study we made a saliva pretreatment plan for cytokines and amylase assays, also we tried to understand the strategy of mechanism associations between different water drinking behavior, the condition of oral immune protection and stress factors.
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Children's Menu Diversity: Influence on Fluoride Absorption and Excretion.
Cavalli, AM, Flório, FM
The journal of contemporary dental practice. 2018;(1):30-36
Abstract
AIM: The aim of this study is to determine the influence of children's menu diversity on the absorption and excretion of fluoride. MATERIALS AND METHODS The experimental, longitudinal, quantitative study was carried out in a city without fluoridation in water supply. A total of 16 adult volunteers (>63.9 kg) participated in the study who, after a 12-hour fast, ingested two types of children's meals, whose quantity and diversity were determined after weighing the meals best consumed by children at a kindergarten in Campinas, Sao Paulo: Simple child meal (SCM; n = 8) and hearty child meal (HCM; n = 8). The fluoride gel residual after professional application (12,300 ppm, 30.75 mg F, pH = 4.65) was simulated 15 minutes after feeding. Saliva samples (in time intervals of 0, 15, 30, and 45 minutes and 1, 2, 3, 4, 6, and 12 hours after ingestion of the fluorine solution) and urine of the volunteers were analyzed at 24 hours. Fluoride concentrations were determined using a selective ion electrode. Data were analyzed by analysis of variance for repeated measurements (PROC MIXED)/Tukey-Kramer. RESULTS The concentrations of fluoride in saliva at 0 and 15 minutes and after 6 hours were the same between groups (p > 0.05). From 30 minutes to 4 hours after ingestion, the SCM group showed a higher concentration of fluoride in the saliva, which has a higher absorption (p < 0.05). The fluoride concentration in the urine did not differ between groups at both collection times (p > 0.05), and for both, the fluoride concentration in the urine increased in the final measurement (p < 0.05). CONCLUSION The children's menu diversity influenced the absorption of fluoride so that the topical application of fluoride should be performed in infants fed preferably after the fuller diet and following the established guidelines to ensure the safety of the procedure. CLINICAL SIGNIFICANCE Knowledge of the influence of the children's menu diversity on fluoride metabolism after professional application is important so that the actions of fluoride therapy may be planned in a safer manner and be based on the reality of the universe of children.
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A Randomized Single Dose Parallel Study on Enhancement of Nitric Oxide in Serum and Saliva with the Use of Natural Sports Supplement in Healthy Adults.
Jacob, J, Gopi, S, Divya, C
Journal of dietary supplements. 2018;(2):161-172
Abstract
Sports supplements that stimulate the production of nitric oxide (NO) are widely promoted agents in the sports nutrition domain, and nitric oxide plays an important role to enhance the cardiovascular and physical fitness of the sports participants. The purpose of the study is to investigate whether oral intake of a sports nutritional supplement (Fitnox) is able to increase nitrate (NO3-) and nitrite (NO2-) levels in blood serum and saliva of healthy adults. Fitnox is a unique blend of Kaempferia parviflora methoxy flavones, pomegranate peel polyphenols, and Moringa oleifera leaf saponins. Twenty-four healthy male adults were equally divided and underwent the double-blind, placebo-controlled clinical trial with a single oral dose of sports nutrition formulation (250 mg capsules); blood and saliva samples were analyzed at different time intervals by high-performance liquid chromatography (HPLC). After administration of Fitnox (250 mg capsule as single dose), NO3- and NO2- levels in serum and saliva were found to be significantly higher (p <.05) than in the placebo group in 24 hours. Pharmacokinetic parameters such as the area under the plasma concentration-time curve extrapolated to infinity (AUC0-inf), AUC calculated to the last measured concentration (AUC0-t), maximum drug serum concentrations (Cmax), time of maximum concentration in serum observed (Tmax), and time required for the concentration of the drug to reach half of its original value (Thalf) were also statistically significant (p <.05) compared with the placebo. The results indicate that a single oral dose of Fitnox is able to increase the NO3- and NO2- levels considerably in the body relative to placebo for at least 12 hours. Therefore, Fitnox can improve the overall performance of sport participants and enhance physical endurance.
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Salivary functions in mastication, taste and textural perception, swallowing and initial digestion.
Pedersen, A, Sørensen, CE, Proctor, GB, Carpenter, GH
Oral diseases. 2018;(8):1399-1416
Abstract
Saliva exerts multiple functions in relation to the initial digestive processes taking place in the upper parts of the gastrointestinal tract. Ingestion of food and beverages, in turn, is a strong stimulus for secretion of saliva with a differential composition depending on the neuronal stimulation pattern. This review paper provides insight into the mechanisms by which saliva acts in relation to taste, mastication, bolus formation, enzymatic digestion and swallowing. Also, the protective functions of saliva including maintenance of dental and mucosal integrity will be discussed as they indirectly influence the digestive process. The final part of this study focuses on the implications of xerostomia and salivary gland dysfunction on gastrointestinal functions.
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A cluster randomized pilot trial of a tailored worksite smoking cessation intervention targeting Hispanic/Latino construction workers: Intervention development and research design.
Asfar, T, Caban-Martinez, AJ, McClure, LA, Ruano-Herreria, EC, Sierra, D, Gilford Clark, G, Samano, D, Dietz, NA, Ward, KD, Arheart, KL, et al
Contemporary clinical trials. 2018;:47-55
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Abstract
Construction workers have the highest smoking rate among all occupations (39%). Hispanic/Latino workers constitute a large and increasing group in the US construction industry (over 2.6 million; 23% of all workers). These minority workers have lower cessation rates compared to other groups due to their limited access to cessation services, and lack of smoking cessation interventions adapted to their culture and work/life circumstances. Formative research was conducted to create an intervention targeting Hispanic/Latino construction workers. This paper describes the intervention development and the design, methods, and data analysis plans for an ongoing cluster pilot two-arm randomized controlled trial comparing an Enhanced Care worksite cessation program to Standard Care. Fourteen construction sites will be randomized to either Enhanced Care or Standard Care and 126 participants (63/arm) will be recruited. In both arms, recruitment and intervention delivery occur around "food trucks" that regularly visit the construction sites. Participants at Enhanced Care sites will receive the developed intervention consisting of a single face-to-face group counseling session, 2 phone calls, and a fax referral to Florida tobacco quitline (QL). Participants at Standard Care sites will receive a fax referral to the QL. Both groups will receive eight weeks of nicotine replacement treatment and two follow-up assessments at three and six months. Feasibility outcomes are estimated recruitment yield, barriers to delivering the intervention onsite, and rates of adherence/compliance to the intervention, follow-ups, and QL enrollment. Efficacy outcomes are point-prevalence and prolonged abstinence rates at six month follow-up confirmed by saliva cotinine <15 ng/ml.
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Short-term changes in daily movement behaviour influence salivary C-reactive protein in healthy women.
Truba, TN, Doan, J, Currie, CL, Copeland, JL
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 2018;(8):854-856
Abstract
This study assessed the effect of changing daily movement behaviour on C-reactive protein (CRP) measured in saliva. Two groups of women either reduced daily movement or increased physical activity for 10 days. Salivary CRP increased by 31% in the sedentary group (0.378 ± 0.596 to 0.487 ± 0.793 μg·L-1) and decreased by 22% in the active group (0.414 ± 0.640 to 0.259 ± 0.284 μg·L-1). These results suggest short-term changes in daily movement behaviour can affect salivary CRP, a marker of systemic inflammation.
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Effect of tablets containing probiotic candidate strains on gingival inflammation and composition of the salivary microbiome: a randomised controlled trial.
Keller, MK, Brandsborg, E, Holmstrøm, K, Twetman, S
Beneficial microbes. 2018;(3):487-494
Abstract
The aim of the study was to investigate clinical and microbial effects of probiotic candidate strains in patients with moderate gingivitis. The null hypothesis was that the clinical measurements with treatment would not differ from placebo. 47 adult patients were enrolled in a randomised placebo-controlled trial with a 4-week intervention of tablets containing a mix of Lactobacillus rhamnosus PB01, DSM 14869 and Lactobacillus curvatus EB10, DSM 32307 or placebo. Clinical examinations and samplings were done at baseline and after 2, 4 and 6 weeks. The clinical endpoints were general bleeding on probing (BOP), general plaque index (PI) and flow of gingival crevicular fluid (GCF). In addition, the concentration of selected cytokines (interleukin (IL)-1β, IL-6, IL-8, IL-10, tumour necrosis factor alpha (TNF-α)) in GCF was determined with multiplex immunoassays. The profiles of the salivary microbiome were analysed with Next Generation Sequencing (NGS) and qPCR. In contrast to the placebo group, there was a significant reduction in BOP and amount of GCF (P<0.05) after 4 weeks in the probiotic test group when compared with baseline. The general PI was less affected although there was a tendency of decreased plaque levels in the probiotic group (P=0.05-0.09). The cytokines were unaffected by the intervention as well as the salivary microbiome. The Shannon index showed no significant differences between the groups or alterations over time. The occurrence of both probiotic strains increased in saliva of the test subjects during the intervention but returned to baseline levels within 2 weeks. Although a marked improvement in gingival health was recorded in the probiotic group, the null hypothesis could not be rejected.
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Salivary biomarkers and cardiovascular disease: a systematic review.
Gohel, V, Jones, JA, Wehler, CJ
Clinical chemistry and laboratory medicine. 2018;(9):1432-1442
Abstract
BACKGROUND The purpose of this systematic review is to summarize the literature examining associations between salivary biomarkers and cardiovascular disease (CVD) status. CONTENTS An advanced search was conducted using MeSH terms related to salivary biomarkers and CVD, and entered into the PubMed, Web of Science, and Google Scholar search databases. Four hundred and thirty-three records were narrowed to 22 accepted articles. Included titles were assessed for quality using the Newcastle-Ottawa scale, and ranked into categories of low, moderate, or high. SUMMARY A total of 40 salivary biomarkers were analyzed among accepted articles. The most studied markers were salivary creatine kinase isoform MB, C-reactive protein (CRP), matrix metalloproteinase-9, troponin I, myeloperoxidase, myoglobin, and brain natriuretic peptide. Salivary CRP provided the most consistent trends. Statistically significant increases of salivary CRP were present with CVD in every study that analyzed it. The remaining six markers demonstrated varying patterns. OUTLOOK Existing studies provide insufficient data to draw definitive conclusions. Current research shows that there is an association between some salivary biomarkers and CVD, but the details of existing studies are conflicting. Despite inconclusive results, the diagnostic potential of saliva shows promise as a non-invasive means of cardiovascular risk assessment.
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Sialendoscopy enhances salivary gland function in Sjögren's syndrome: a 6-month follow-up, randomised and controlled, single blind study.
Karagozoglu, KH, Vissink, A, Forouzanfar, T, Brand, HS, Maarse, F, Jager, DHJ
Annals of the rheumatic diseases. 2018;(7):1025-1031
Abstract
OBJECTIVES To assess the effect of sialendoscopy of the major salivary glands on salivary flow and xerostomia in patients with Sjögren's syndrome (SS). METHODS Forty-nine patients with SS were randomly assigned to a control group (n=15) and two intervention groups: irrigation of the major glands with saline (n=16) or with saline followed by triamcinolone acetonide (TA) in saline (n=18). Unstimulated whole saliva flow (UWS), chewing-stimulated whole saliva flow (SWS), citric acid-stimulated parotid flow (SPF), Clinical Oral Dryness Score (CODS), Xerostomia Inventory (XI) score and the European League Against Rheumatism (EULAR) SS Patient-Reported Index (ESSPRI) were obtained 1 week (T0) before, and 1 (T1), 8 (T8), 16 (T16) and 24 (T24) weeks after sialendoscopy. RESULTS Median baseline UWS, SWS and SPF scores were 0.14, 0.46 and 0.22 mL/min, respectively. After intervention, significant increases in UWS and SWS were observed in the saline group (at T8 (P=0.013) and T24 (P=0.004)) and the saline/TA group (at T24 (P=0.03) and T=16 (P=0.035)). SPF was increased significantly in the saline/TA group at T24 (P=0.03). XI scores declined after sialendoscopy in both intervention groups. Compared with the control group, CODS, XI and ESSPRI improved in the intervention groups. UWS, SWS and SPF were higher in the intervention groups compared with the control group, but these differences were not significant except for SPF in the saline/TA group at T24 (P=0.005). CONCLUSIONS Irrigation of the major salivary glands in patients with SS enhances salivary flow and reduces xerostomia up to 6 months after sialendoscopy.
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Effects of placentophagy on maternal salivary hormones: A pilot trial, part 1.
Young, SM, Gryder, LK, Cross, C, Zava, D, Kimball, DW, Benyshek, DC
Women and birth : journal of the Australian College of Midwives. 2018;(4):e245-e257
Abstract
BACKGROUND Recent studies show that human placenta, processed and encapsulated for postpartum consumption, contains a host of trace minerals and hormones that could conceivably affect maternal physiology. Our objective was to investigate whether salivary hormone concentrations of women ingesting their own encapsulated placenta during the early postpartum differed from those of women consuming a placebo. METHODS Randomly assigned participants (N=27) were given a supplement containing either their dehydrated and homogenized placenta (n=12), or placebo (n=15). Saliva samples were collected during late pregnancy and early postpartum. Samples of participants' processed placenta, and the encapsulated placebo, were also collected. Hormone analyses were conducted on all samples utilizing liquid chromatography-tandem mass spectrometry. RESULTS There were no significant differences in salivary hormone concentrations between the placenta and placebo groups post-supplementation that did not exist pre-supplementation. There were, however, significant dose-response relationships between the concentration of all 15 detected hormones in the placenta capsules and corresponding salivary hormone measures in placenta group participants not seen in the placebo group. The higher salivary concentrations of these hormones in the placenta group reflects the higher concentrations of these hormones in the placenta supplements, compared to the placebo. CONCLUSIONS Some hormones in encapsulated placenta lead to small but significant differences in hormonal profiles of women taking placenta capsules compared to those taking a placebo, although these dose-response changes were not sufficient to result in significant hormonal differences between groups. Whether modest hormonal changes due to placenta supplementation are associated with therapeutic postpartum effects, however, awaits further investigation.