Procaryotic expression of phosphorylated tegument protein pp65 of human cytomegalovirus and application of recombinant peptides for immunoblot analyses.

Journal of clinical microbiology. 1990;28(6):1229-35

Plain language summary

With the rising epidemic of obesity and related chronic illnesses, it is important to understand the body fat distribution i.e. Intra-abdominal adipose tissue (IAAT) and subcutaneous adipose tissue (SAT) and its clinical implications. Body fat stores and releases energy, but excess abdominal fat and ectopic fat may potentially have harmful effects on various bodily metabolic functions and is also a major metabolic risk factor. This study used magnetic resonance imaging and proton magnetic resonance spectroscopy techniques to enable a more precise quantitative measure of the adipose tissue. The study aimed to calculate the reference range for total and regional adipose tissue using anthropometric variables and MRI and MRS scanning methods. A large heterogeneous cohort of 477 volunteers, with no age restriction was recruited. Body mass, height, waist circumference and hip circumference and adipose tissue distribution were measured for each individual taking part in the study. Data suggests that whilst both IAAT and SAT are correlated to metabolic risk factors, IAAT is more strongly associated with an adverse metabolic risk profile after accounting for anthropometric indices.The anthropometric measures which provided the strongest correlation with individual adiposity was body mass index in women and waist circumference in men.

Abstract

The tegument of human cytomegalovirus (HCMV) contains a phosphorylated protein of 65 kilodaltons, termed pp65, which was reported to carry significant epitopes for the stimulation of the humoral immune response during natural infection. A monoclonal antibody directed against this protein was used to screen a lambda gt11 cDNA library for recombinant polypeptides. Two DNA fragments from purified lambda clones and one fragment from genomic DNA were used for cloning in a bacterial high-level expression vector. The resulting fusion proteins were tested for their reactivity with a panel of monoclonal antibodies directed against pp65 and with polyspecific anti-HCMV rabbit antisera. The binding site for all the monoclonal antibodies tested was found to be contained in one of the recombinant proteins with a viral portion of 26 amino acids. Immunoblot analyses with HCMV-positive human sera revealed that pp65 alone is not a reliable antigen for serodiagnosis but may be very useful in combination with other HCMV proteins.

Lifestyle medicine

Fundamental Clinical Imbalances : Hormonal ; Immune and inflammation
Patient Centred Factors : Mediators/Adipose tissue
Environmental Inputs : Diet
Personal Lifestyle Factors : Nutrition ; Environment
Functional Laboratory Testing : Imaging

Methodological quality

Allocation concealment : Not applicable

Metadata

Nutrition Evidence keywords : Body fat ; Visceral fat